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Image Search Results
Journal: Frontiers in Neuroscience
Article Title: Modeling Alzheimer’s disease related phenotypes in the Ts65Dn mouse: impact of age on Aβ, Tau, pTau, NfL, and behavior
doi: 10.3389/fnins.2023.1202208
Figure Lengend Snippet: Total Tau and pTau S396 protein level in Ts65Dn and 2N mouse brain total homogenate and insoluble fraction at different ages by Meso Scale Discovery and WB. (A) Schematic representation of the fractions used for evaluation. The total homogenate was used for measuring total Tau and pTau levels. P3-SinT was used for the Sarkosyl insoluble Tau fraction. (B) Total Tau measured by Meso Scale Discovery. Age had a significant effect on levels of total Tau ( p < 0.0001). (C) Total Tau as measured by WB. Age had a significant effect ( p = 0.0218) as did genotype ( p = 0.0218). (D) pTau S396 protein level in total brain homogenates of 2N and TS65Dn mice at different ages as measured by Meso Scale Discovery. Age ( p < 0.0001) and genotype ( p = 0.0228) were significant factors for pTau S396 levels. (E) WB evaluation of pTau S396 protein level in total brain homogenates of 2N and TS65Dn mice at different ages measured. Age ( p < 0.0001) was a significant factor for pTau S396 levels. The concentrations of pTauS396 obtained by Meso Scale Discovery were normalized on the total protein concentration, while the results obtained by WB were normalized on the total protein concentration and expressed as the corrected area. (F) pTau S396 protein level in Sarkosyl-insoluble fraction of the 13- and 16-month-old cohort measured by WB. ANOVA indicated a significant effect of genotype ( p = 0.0066), age ( p = 0.0024), and interaction of age and genotype ( p = 0.0066). (G) pTau S396 protein level in Sarkosyl-insoluble fraction of the 16-month-old cohort measured by Meso Scale Discovery. Results are expressed as individual values and mean ± SD.
Article Snippet: Post hoc Tukey’s multiple comparisons tests identified significant increases in
Techniques: Protein Concentration